Comparative phenotypic analysis of JAY270 and S288c. (A) Sugar cane extract fermentation assays (∼18% sucrose). The ethanol concentration shown is the average reached in four fermentation assay repetitions, each comprised of four consecutive 15 h of fermentation at 30°C with cell recycling (see Methods for details). (B) Reactive oxygen (menadione) and temperature effects on colony growth. Tenfold serial dilutions of saturated cultures spotted (5 μL) in rich medium (YPD) containing various concentrations of menadione were incubated at the indicated temperatures and scanned after the indicated incubation period. The S288c-isogenic control strain used in A was the BY4741 haploid and in B was the JAY309 diploid.
