Effect of glucocorticoid receptor knockdown on dexamethasone response. (A) Western blot showing the effect of siRNA-mediated knockdown on GR protein levels. (B) Gene expression response of 46 DEX-responsive genes to 5-h 100 nM DEX treatment in cells transfected either with control (white bars) or GR-targeting (black bars) siRNA pools. Three independent knockdown experiments were performed for each of two siRNA pools. Error bars, SEM across all six measurements. Measurements for each siRNA pool are available in Supplemental Figures S6 and S7. (C) Box plot showing overall effect of siRNA-mediated knockdown of the GR on the gene expression response for DEX-induced and DEX-repressed genes. Whiskers indicate 10%-90% range.
