Direct and indirect DNA binding by Ste12 and Tec1. Ste12 and Tec1 are both involved in two developmental processes: filamentation (induced by treatment with butanol, as in the BUT14 condition) and mating (induced by treatment with the alpha pheromone, as in the Alpha condition). (A) During filamentation, the Tec1-Ste12–Dig1 complex binds DNA through Tec1. Our method correctly identifies Tec1 as the only significantly enriched TF in the ChIP-chip experiments where filamentation occurs. (B) During mating, the Ste12–Dig1–Dig2 and Ste12–Tec1–Dig 1 complexes bind DNA through Ste12. Our method correctly identifies Ste12 as the only significantly enriched TF in the ChIP-chip experiments where mating occurs.
