(A) PCNEs associated with the Sox14/21 gene family. Each genomic region is 204 kb long. Genes are shown as arrows indicating the direction of transcription. Black boxes indicate exons. The small single-exon Sox14/21 genes are labeled with red triangles. PCNEs are shown as colored lines; the colors and the numbers above the PCNEs indicate the group membership of each PCNE and reveal conservation of PCNE order. Neighboring genes that may represent conserved syntenic relationships are labeled; unlabeled genes are not syntenically conserved. A maximum likelihood phylogenetic tree of the Sox14/21 proteins is shown on the right. (B) A multiple alignment of a portion of the Sox14/21 group 1 PCNEs, with conserved binding motifs highlighted in pink. Bf, amphioxus; Tr, fugu; Dr, zebrafish; Mm, mouse.
