Figure 5.
CDR3β nucleotide length distribution and sequence composition of the most abundant CDR3β length. To explore CDR3β length variation we used a precise length criterion, defined as all bases between the last cysteine of TRBV and the phenylalanine in the TRBJ segment motif FGXG. Of the 33,664 total clonotypes, 30,366 could be classified in this manner, and the length distribution of this subset was plotted (A). The most frequently observed length was 45 nt. For the subset of clonotypes with 45 nt CDR3β sequences, we created logos for the nucleotide (B) and inferred amino acid (C) composition, using WebLogo (Crooks et al. 2004).
