(A) Representation of the TCRβ locus at human chromosome 7q34. The TCRβ locus spans 620 kb and includes over 50 TRBV genes (green) belonging to 30 subgroups. There are two TRBC genes (light blue) each downstream from a TRBD (dark blue) and six or seven TRBJs (yellow). Recombination first occurs between TRBJ and TRBD genes, followed by recombination to a TRBV gene. (Red lines) Addition of nontemplated bases. After transcription, intervening sequences are spliced out so that a TRBC is adjacent to the recombined V-D-J sequence. Gene width and distances are not to scale. (Red asterisks under TRBC) Location of primers used for 5′-RACE and PCR reactions. Refer to Supplemental Figure 1A for primer locations. A detailed locus map can be obtained from IMGT (www.imgt.org/textes/IMGTrepertoire). (B) Flowchart illustrating 5′-RACE and Illumina library construction. For more detail, please refer to Methods and Supplemental Figure 1.
