Deterministic protein inference for shotgun proteomics data provides new insights into Arabidopsis pollen development and function

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Table 1.

Summary of identified spectra, peptides, and proteins by evidence class

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Table 1.
  • The total number of spectra for each respective evidence class, the distinct peptides (after validation of the single hit protein identifications), and the distinct number of protein identifications per class are shown. The latter number is conservative in that only gene models of class 2 are listed that are not already implied by a higher information content-based identification (classes 1a, 1b). For class 3b, we cannot give an exact protein count, the smallest protein set explaining these peptides amounts to 132 proteins, whereas the maximum number of proteins could include up to 2557 proteins.

  • aInformation-rich peptides implied by a single mass spectrum (classes 1a−3a) were manually validated; the final number of spectra post-validation is provided in column 3.

  • bA minimum number of proteins implied by class 3b peptides is shown.

This Article

  1. Genome Res. 19: 1786-1800

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