Validated single peptide hit proteins tend to be shorter proteins of varying abundance. 2D density plots of protein length and predicted protein abundance based on FCF values for proteins identified by a validated single peptide hit (A, red), and proteins identified by a rejected single peptide hit (B, blue). (C) A different view of the previous plots (red, areas of relative overrepresentation of the validated single hit proteins; blue, areas where false-positive identifications are overrepresented) shows that validated single peptide hit identifications tend to be shorter proteins. Very short proteins that were successfully identified are preferentially higher abundant proteins.
