Table 1.
Comparison of 2S rRNA depletion by immobilized oligonucleotide hybridization/beta-elimination and cation-exchange chromatography
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Seitz et al. (2008) cloned 18–30-nt RNA libraries from S2 cells and heads RNA depleted of 2S rRNA with an immobilized oligonucleotide, either before (non-beta [NB]) or after (B) beta-elimination. Cloning of 18–34-nt RNA libraries by the Q-column strategy also yielded effective depletion of 2S rRNA reads.
