Sequence changes in the origin of DNAH10OS from noncoding DNA. As in Figures 2 and 3: (A) region of conserved synteny between human and chimp chromosomes 12. One gene in this region, DNAH10OS, had no BLASTP hits in any other genome and is shown in green. (B) Multiple sequence alignment of the gene sequence of DNAH10OS and similar nucleotide sequences from the syntenic location in chimp and macaque. If the ORF began at the same position as the human start codon (note the start codon is present in chimp but absent in macaque), the macaque hypothetical protein sequence would be very different from the human protein due to frameshifts and would terminate at the stop codon indicated in yellow. The arrow indicates the location of a 10-bp indel shared by chimp and macaque that would result in premature termination irrespective of the other disablements. (C) The disabler is also shared by gorilla, orangutan, and gibbon indicating that this is a human-specific 10-bp insertion.
