Identification of miniature inverted-repeat transposable elements (MITEs) and biogenesis of their siRNAs in the Solanaceae: New functional implications for MITEs

MiS small RNAs detected in Solanum and Nicotiana species by Northern blot hybridization. Forty micrograms of small RNA-enriched from total RNA isolated from Solanaceae species was size separated using a 20% acrylamide gel, blotted, and hybridized with MiS-derived probes (see Supplemental Table S3.1 for a description of probes). (A) Hybridization probe for (upper panel) Solanum-MiS17 probe; (lower panel) Nicotiana-MiS17; (B) Nicotiana-MiS1 probe; (C) Solanum-MiS15 probe; (D) Solanum-MiS5 probe; (E) Solanum-MiS8 probe; (F) Os-Ditto probe; (G) MIR165. An EtBr stain is shown for each gene as a sample loading control. (At) A. thaliana, Col-0; (Os) O. sativa Nipponbare; (Nb) N. benthamiana; (Nt^S) N. tabacum, SR1; (Nt^TG34) N. tabacum, SR1:NN; (Ng) N. glutinosa; (St^KE) S. tuberosum, Kennebec; (St^PI) S. tuberosum, Pentland Ivory; (Sd) S. demissum; (Sb) S. bulbocastanum; (St^L) S. tuberosum, Katahdin leaves; (St^F) S. tuberosum, Katahdin flowers; (Sl^L) S. lycopersicum VF36 leaves; (Sl^F) S. lycopersicum, VF36 flowers.