Examples of FISH experiments on Hylobates lar metaphases using human BAC clones as probes. (a) Three examples of inversions, occuring in Hylobatidae ancestor, in which one of the two breakpoints fall in the centromeric/pericentromeric region. The figure shows the signals of the splitting BAC defining the euchromatic breakpoint. The code, in parenthesis on the right of each BAC name, refers to Supplemental Tables ST1 and ST2, where the BAC precise position on the human sequence is also reported. (b) The two BACs RP11-380J21 (red signal) and RP11-183N22 (green signal) are located, in humans, at chr3:64,053,486–64,212,751 and chr3:4,328,222–4,493,696, respectively, suggesting a rearrangement with respect to humans. On the contrary, the rearrangement occurred in the lineage leading to humans.
