miR1429.2 is a putative mirtron. (A) Pre-miR1429.2 (shown in capital letters; adjacent exon sequences are shown in lowercase letters; miR1429 is underlined) with aligned small RNAs. The mature miRNA (miR1429.2 in red) and its miRNA* (in blue), and the termini of the intron (vertical pink lines), are indicated with the predicted secondary structure shown in bracket notation. Positions of the annotated splicing donor and acceptor were supported by cDNA sequence AB101650 (sequences flanking the intron are shown in italic at the first row). The 3′ terminal nucleotide in the last two small RNAs, which do not match the genomic sequence, might be generated by untemplated addition. (B) Predicted secondary structure of pre-miR1429.2 showing 2-nt 3′ overhangs.
