Figure 4.
Frameshift generated by sequencing error. In top panel, the nucleotide sequence for gene SO0590 is shown in red, the amino acid sequence of the protein is shown in green, and the amino acid sequences of the three translated frames are shown in black. Peptides identified by mass spectrometry are marked in blue (surrounded by boxes). The middle panel shows the ClustalW alignment with other Shewanella species in the region where frameshift occurs. The erroneous insertion of an extra “t” stands out in the alignment. The bottom panel indicates that both peptides fall in the original frame if the extra nucleotide is removed.
