Properties of Fis-binding regions. (A) Classification of Fis-binding regions based on the binding patterns. (i) Fis bound near the promoter of brnQ is a member of the IG1 class, which encompasses Fis bound near promoter regions of the currently annotated genes. (ii) Fis bound between hemN and glnG is a member of the IG2 class, which encompasses Fis bound within the region of two divergently transcribed genes. (iii) Fis bound within the nusA gene is a member of the ORF class, which binds within the open reading frame of annotated genes. (B) Distribution of Fis binding between the three classes. (C) Many of the Fis-binding regions (IG1, IG2, and ORF) are also occupied by RNAP and σ70.
