
Coimmunoprecipitation of full-length and IΔ1 type IAP proteins. HEK293 cells were transfected with pFLex expressing the full-length type IAP protein and pDE1ex-HA expressing HA-tagged IΔ1 type IAP protein. pBluescriptII was mock transfected as a control. Proteins immunoprecipitated with anti-HA antibody or with control antibody of the same subclass (anti-Oct3/4 [currently known as POU5F1]) were blotted with antiserum that detects full-length type IAP-derived Gag or with the anti-HA antibody. Migration of immunoprecipitated proteins (asterisk) is retarded compared to the input under our conditions.











