Parent-of-origin analysis of genes showing ASE in a replicate set. (A) NLRP2 demonstrates preferential expression of the maternal allele in placentas of eight different individuals. (B) OSBPL1A demonstrates preferential expression of the maternal allele in placentas of seven (shown) out of 10 different individuals. In both A and B, the first row demonstrates the simplex files for the three possible genotypes of the pyrosequencing assay. The next two rows demonstrate the pyrosequencing raw data files for the gDNA of the mother (homozygous) and the offspring (heterozygous), respectively. The final rows demonstrate the ASE (in triplicate) from cDNA of the cases and the corresponding RT− control for each assay. In all cases, the maternal allele (red) is preferentially expressed compared with the paternal allele (blue). The columns represent individual sample sets.
