High-throughput biochemical analysis of in vivo location data reveals novel distinct classes of POU5F1(Oct4)/DNA complexes

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Figure 3.
Figure 3.

Changes in oligonucleotide enrichment throughout an POU5F1 SELEX experiment. (A) Enrichment scores for each round of SELEX were binned and graphed as a histogram. (B) Average enrichment scores were ranked with relevant oligonucleotides marked on the percentile bar. Gel shift assay was repeated for isolates cloned from selected (C) and unselected (D) fractions. (E) Microarray images corresponding to pool/pool and pool/round1 are drawn below the gel lane for each oligonucleotide.

This Article

  1. Published in Advance January 22, 2008, doi: 10.1101/gr.072942.107 Genome Res. 18: 631-639

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