Chromosomal mapping of predicted RNA structures. (A) Chromosomal location of the initial 855 RNAz features predicted on P. falciparum genome. Each colored bar represents a feature, plotted along the 14 chromosomes as indicated. Keys to the colors and symbols are shown in the inset box. (B) Northern blots showing ring (R) and pigmented trophozoite (T) stage total RNA, hybridized with reverse-complement oligonucleotide probes to predicted transcripts, for three of the 78 RNAz candidates. Size references indicated in nucleotides (as derived in Supplemental Fig. S5). Candidates 2846 and 2132 are novel predicted snoRNAs, with transcription confirmed by microarray. Candidate 2136 is unique to the Northern blot screen, as no PFSANGER microarray probe covered this locus.
