Reciprocal exchange of telomeres and chromosome arms in yeast. (A) Depiction of native chr 5 (red) and chr 6 (blue). (B,C) Overview of the telomere swap (B) and chromosome arm swap (C) (see Supplemental Fig. 5 for detailed explanation). Black bars underneath the chromosomes indicate the annealing positions of the probes used for Southern hybridization. (D) Total intact genomic DNA in the form of chromosomes was isolated from the indicated strains before and after the exchange (swap). Chromosomes were separated by CHEF gel electrophoresis and stained with ethidium bromide. Southern blot analysis was performed with probes indicated in (A–C). (Lane 1) GA-3146, (lane 2) GA-1607, (lane 3) GA-1459, (lane 4) GA-1095, (lane 5) GA-3747, (lane 6) GA-3746, (lane 7) GA-3749, (lane 8) GA-3748, (lane 9) GA-3747, (lane 10) GA-3746, (lane 11) GA-3749, (lane 12) GA-3748.
