Expression of predicted ncRNA candidates by RT-PCR and Northern blot analysis. (A) Strand-specific RT-PCR analysis of ncRNA candidates on human RNA pools (see Methods). β-Actin was used as control, yielding PCR products in the presence of reverse transcriptase (RT+), but not in its absence (RT−). (B) Tissue-specific expression of ncRNA candidates as evaluated by RT-PCR analysis of human RNA samples. The same β-actin controls as for A were used. (C) Expression of ncRNA candidates within the human CNS as evaluated by RT-PCR analysis. The same β-actin controls as for A and B were used. (D) Expression of ncRNA candidate #6 as evaluated by Northern blotting of human RNA samples from 11 tissues.
