(A) Chromosome walk from the Y-specific AMELY gene across the bovine PAB. BACs are sorted by contigs as retrieved from the BAC-based fingerprint map of the bovine genome (http://www.bcgsc.ca/platform/mapping/bovine). Only BACs relevant in the context of this study are shown. (Blue dots) Y-specific BAC end sequences, (red dots) pseudoautosomal BAC end sequences. Dotted lines connect BAC ends used as probes in filter hybridization and resulting positive BAC clones. Sequenced BACs are shown as finished (white bars) or draft (gray bars). (White bar with dotted contour) Sequenced PCR product and plasmids connecting E0064F17 and E0232B11. (Arrows) Interval encompassing the bovine PAB. The identifiers of the BACs used as probes in the fluorescence in situ hybridization (FISH) experiment are labeled (red and green, respectively). (B) Representative FISH results obtained with the Y-specific E0232B11 (red) and pseudoautosomal H0202L11 (green) BACs on female (left panel) and male (right panel) metaphases, respectively.
