Use of shotgun proteomics for the identification, confirmation, and correction of C. elegans gene annotations

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Figure 4.
Figure 4.

Identification of a novel coding sequence by shotgun proteomics. Three unique peptides were identified in the genomic region 16,652,022–16,654,397 on the X chromosome. This genomic region represents a new ORF from the new GeneFinder prediction set. There are no gene models predicted in this region in WormBase WS150; however, several SAGE tags confirming this gene model have been added since WS150. A mass spectrum from the peptide SPASGSALLDLLSR is shown.

This Article

  1. Genome Res. 18: 1660-1669

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