Deep sequencing of tomato short RNAs identifies microRNAs targeting genes involved in fruit ripening

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Figure 3.
Figure 3.

Differentially expressed tomato short RNAs. Probes specific to potential miRNAs (tom72, NGM3, tom177, tom179, tom122, tom40, and tomtar3) or short RNAs that could not be mapped to the available genome sequence but cloned many times (top15, top12, top9, and top11) were hybridized to the same membranes shown on Figure 2. Sequences of short RNAs are provided in Supplemental Figure 2. The U6-specific probe shows equal loading. Different size fruits were used for RNA extraction: F1, 1–3 mm; F2, 5–7 mm; F3, 7–11 mm; F4, 11–14 mm. Size markers on the left are 24- and 19-nt RNA oligonucleotides.

This Article

  1. Genome Res. 18: 1602-1609

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