TetraNR abundance in the human genome for tracts with ≥8 units, Ta values, and DNA structure
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The columns are as follows: Column 1, number of tetraNRs ≥8 units in the human genome; column 2, unique tetraNR sequence; column 3, sequence of the forward oligonucleotides; column 4, annealing temperature (Ta) for the forward oligonucleotide sequences; column 5, structure of the forward oligonucleotides; columns 6–8, same as columns 3–5, respectively, but for the reverse oligonucleotide sequences.
aSelf-complementary sequence with no staggered ends for which Ta measurements for the forward and reverse oligonucleotide sequences are identical.
bSelf-complementary sequence with staggered ends for which Ta measurements for the forward and reverse oligonucleotide sequences are not identical.
cSequences studied by Ta analyses with variable salt concentration assay conditions and/or by circular dichroism (CD).
dStructure in the presence of 100 mM K+ and 10 mM Mg2+. Other structures are possible at different metal ion concentrations (Supplemental material).
(N) Less than 8% total hypochromicity during both the heating and cooling steps without a detectable first-derivative peak; (S) greater than 8% total hypochromicity during either the heating or cooling steps without a detectable first-derivative peak; (C) single-stranded coil; (H) hairpin; (Q) quadruplex.
