Multiple copies of chromosome 19 CNVs are tightly linked and result in altered gene expression. (A) FISH using a fosmid clone from the region hybridized to metaphase spreads from a DupB6/BALB F1 animal. Signal observed in the four chromatids of two homologous chromosomes corresponds to a single location on mouse chromosome 19. (B) Fgfbp3 and Ide expression are increased in animals carrying gene duplications. Expression of Fgfbp3 is significantly (P < 0.001) increased in spleen, but not affected in brain. Expression of Ide is significantly increased in both spleen (P < 0.001) and brain (P < 0.01). The graph depicts the mean±one standard deviation for data from four to six replicates from each of seven to eight animals in each group.
