The FR-MARs from the transgenic constructs are associated with the nuclear matrix in stable HeLa cell transfectants. The nuclear matrix-associated DNA isolated from transfected HeLa cells generated using the pNeo01and pNeo04 constructs was hybridized with an oligonucleotide mini-array consisting of Control, a 4q35 DNA fragment that does not associate with the nuclear matrix; Myc, a constitutive S/MAR from the upstream region of the human MYC gene used as a positive control; FR-MAR; and pNeo, a fragment adjacent to FR-MAR in the pNeo plasmid with no homology with human DNA. Association of the c-myc S/MAR to the nuclear matrix was considered as 100%.
