(A) Genomic architecture and patterns of human inheritance of 5S ribosomal DNA gene clusters. Four families, “ARDO,” “PYFI,” “VAGR,” and “THAE” are shown. Relationship codes: (01) daughter, (02) son, (10) mother, (20) father, (40) maternal grandmother, (60) paternal grandmother, (70) paternal grandfather. Siblings of the same sex and generation are additionally designated “A,” “B,” in descending birth order. (Dotted oval) A band not represented in either parent. rDNA cluster lengths are measured in kilobases and calibrated using lambda phage concatemers. (B) PYFI family 5S rDNA structure at high resolution and contrast. Restriction enzymes used to liberate 5S gene clusters from bulk genomic DNA are shown above sets of family samples. (Dotted ovals) Bands not represented in either parent. (Square brackets) Bands of nonstoichiometric signal intensity. (Left) Lambda phage concatemer size references: λ3 = 145,506 bp, λ4 = 194,008 bp, λ5 = 242,510 bp.
