Altered expression of different classes of proteins during post-natal development. The expression of proteins essential for neurotransmission (A) and ATPases (B) significantly increased from p1 to p45. The expression of mitochondrial proteins exhibited two distinct trends in expression during post-natal development. The expression of some mitochondrial proteins significantly increased from p1 to p45 (C), while the expression of other mitochondrial proteins significantly increased from p1 to p20 but then the expression decreased dramatically (D). After the change in protein expression was determined significant in an ANOVA analysis, a Bonferroni post-hoc test was performed. The significant difference in 14N/15N ratios between p1 and p45 for the Bonferroni post-hoc test are represented for A, B, and C by * (P-value < 0.001) and # (P-value < 0.01). The significant difference in 14N/15N ratios between p1 and p20 for the Bonferroni post-hoc test in D are as follows: ADP, ATP carrier protein 1, ATP synthase beta chain, ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1, NADH dehydrogenase, and Ubiquinol-cytochrome c reductase core protein I had P-values < 0.001, and Amine oxidase [flavin-containing] B, Mitochondrial 2-oxoglutarate/malate carrier protein, and Ubiquinol-cytochrome c reductase complex 11-kDa protein had P-values < 0.05. There was no significant difference detected for any of the proteins between the 14N/15N ratios between p1 and p45 in D. The Y-axis represents 14N/15N ratio.
