Quantification of the synaptosomal proteome of the rat cerebellum during post-natal development

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Figure 1.
Figure 1.

Experimental outline. Cerebella were dissected and homogenized from unlabeled (14N) rats at p1, p10, p20, or p45. Homogenates were then mixed in a 1:1 ratio with total brain homogenate from a 15N p45 rat. The synaptosomal fraction was isolated from each mixture, and then this fraction was digested with proteinase K. One hundred micrograms from each developmental period was analyzed three times by MudPIT for a total of 300 μg for each developmental period. From the mass spectra, proteins were identified by SEQUEST, and the 14N/15N ratio for each identified peptide was calculated by Census. The peptide ratios for each protein were compared between the developmental periods.

This Article

  1. Published in Advance August 3, 2007, doi: 10.1101/gr.6375007 Genome Res. 17: 1378-1388

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