Whole proteome analysis of post-translational modifications: Applications of mass-spectrometry for proteogenomic annotation

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Figure 5.
Figure 5.

(A) Alignment of identified peptides (blue) with the intergenic region between TIGR proteins SO_2299 and SO_2300. The starred positions indicate the last codon of SO_2299 (left, chromosome position 2,412,384) and the first codon of SO_2300 (right, chromosome position 2,412,499). The arrow points to the newly postulated translational start site for SO_2300 (infC gene). (B) Nucleotide sequence of the chromosome region between SO_2299 and SO_2300. The first red segment is the C-terminal end of SO_2299, and the second red segment is the N-terminal region of SO_2300. The region covered by the three identified peptides is underlined, and the arrow indicates our suggested start position for SO_2300. (C) A TBLASTN comparison of the proposed new S. oneidensis MR-1 infC N terminus to genome sequences for S. baltica OS155, Shewanella sp. MR-4, S. putrefaciens strains CN32, S. loihica PV-4, Sodalis glossinidius (gi 84778498), and Photobacterium profundum (gi 46913734). The original start position is indicated by the arrow.

This Article

  1. Genome Res. 17: 1362-1377

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