Mitochondrial enzyme activities and protein levels in the SHR progenitor strain and SHR conplastic strain. (A) Activity levels of citrate synthase, NADH cytochrome c reductase (NCCR), succinate cytochrome c reductase (SCCR), and cytochrome c oxidase in liver mitochondria isolated from the SHR progenitor strain (open bars) and SHR conplastic strain (solid bars). The activity of cytochrome c oxidase was significantly lower in the SHR conplastic strain (n = 6) than the SHR progenitor strain (n = 6, P < 0.001). (B) Mitochondrial protein content in the SHR progenitor strain (n = 6) and SHR conplastic strain (n = 6). Results are expressed as relative fluorescence units determined after loading equivalent amounts of total mitochondrial protein for each sample. (NDUFA9) 39-kDa subunit of NADH:ubiquinone oxidoreductase; (ND6) mitochondrial NADH dehydrogenase subunit 6; (UQCRC1) core 1 subunit of ubiquinol:ferricytochrome c oxidoreductase; (COX1) mitochondrial cytochrome c oxidase subunit 1; (COX2) mitochondrial cytochrome c oxidase subunit 2; (COX4I1) cytochrome c oxidase subunit 4 isoform 1; (ATP5B) β-subunit of the F1F0 ATP synthase; (***) P < 0.001; (**) P < 0.01; (*) P = 0.05 in comparisons between the SHR progenitor and conplastic strains by Student’s t-test.
