“Calling cards” deposited in three promoters. The PCR products from three promoters were hybridized to the bar code array. Shown is the ratio of the intensity of hybridization of each bar code to the intensity of hybridization to an LTR probe on the array. (A) In the GAL1–10 promoter, only the Gal4 bar code is enriched. (B) In the HIS4 promoter, only the Gcn4 bar code is enriched. (C) In the PHO5 promoter, only Pho2 and Pho4 bar codes are enriched. (D) When transposition was induced in media rich in phosphate (YPD), the PHO5-specific primers produced no PCR product, but when transposition was induced in cells grown in low-phosphate media the PHO5-specific primers produced abundant PCR products, which contain only Pho4 and Pho2 bar codes, as revealed by hybridization to the bar code array.
