Relative gene expression levels of the SIM genes between promoter-swapped strains and the BY4741 strain
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Note: For real time PCR data, ratio (RM/BY) or (Swap/BY) = 2[-ΔΔCt], ΔΔCt = [ΔCt(RM or Swap) − ΔCt(BY)]. If ratio (Swap/BY) = 1, trans-effect alone; if ratio (Swap/BY) ≠ 1, cis-effect was involved. If ratio (RM/BY) = ratio (Swap/BY), cis-effect alone; if ratio (RM/BY) ≠ ratio (Swap/BY), both cis and trans. If the 95% confidence intervals for RM/BY and Swap/BY have only a minor overlap, we conclude that both cis- and trans-effects were involved. The null hypothesis of Swap/BY = 1 was tested by the two-tail Student’s t-test and so was the null hypothesis of RM/BY = Swap/BY. Each reaction was repeated at least three times from different RNA and cDNA samples.
aSystematic name.
bSignificantly different from 1 (P < 0.05) by t-test.
cSignificantly different from 1 (P < 0.01) by t-test.
