Table 1B.
RNAi-depletion of CENPC and rescue with GFP CENPC1 wild-type and NoLS mutant constructs
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Human 293 cells were depleted of CENPC1 by transfection of CENPC1-87 RNAi oligonucleotide followed by rescue transfection with either wild-type (WT#1) or mutant GFP-CENPC1 (mut#1) carrying mutations at K492, K497, and K498 in the NoLS. The results indicated a positive rescue or increase in cell growth by 18.5% using the wild-type GFP-CENPC1 construct but not with the NoLS mutant construct (+0.2%).
