Depletion of CENPC1 by RNAi and rescues with wild-type and NoLS-mutant GFP-CENPC1 DNA constructs
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Human 293 cells were transfected with three commercially available CENPC1 RNAi oligonucleotides (CENPC1-85, -86, and -87) to knockdown the endogenous CENPC1 in the cells. The decrease in CENPC1 expression level was determined by reverse transcription and real-time PCR 2 d post-transfection (data presented as percentage of expression level compared with that of control cells). The effect of the CENPC1 RNAi knockdown on cell growth was assessed by standard cell count technique. Among the three RNAi oligonucleotides, CENPC1-87 transfection gave the highest reduction of endogenous CENPC1 expression level and the most significant reduction in cell growth as indicated by the decrease in total cell number.
