A model for the assembly of human centromere-associated nucleoprotein components at the interphase nucleolus and mitotic centromere. The nucleolar and centromeric accumulation of CENPC1, INCENP, and/or survivin is dependent on the presence of single-stranded α-satellite RNA. The nucleolus may serve as an interphase storehouse for the sequestration of centromere-associated nucleoprotein components and facilitate the assembly of these components into complexes, prior to their release into the nucleoplasm for relocation, together with additional nucleoplasmic centromere-associated protein components such as survivin, onto the mitotic centromere.
