Novel RefSeq exons verified by RT-PCR
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Forty novel exons are shown that were tested by RT-PCR using RNA derived from ES cells, whole embryo at E14.5, heart, brain, and eye. Exons are indicated that were found only in ES-cell RNA as “ES-only,” those that were absent in ES-cell RNA but present in all other tissues as “ES-absent,” those that were detected in all RNAs tested as “ubiquitous,” those that showed complex on/off patterns and different products in the RNAs tested as “complex,” and those that could not be detected in any of the RNAs tested as “absent.” Overall, 70% of the exons tested could be detected. Moreover, the table separates novel exons according to their location within the gene structure (5′, internal, and 3′ with respect to the annotated gene). For more details, see Supplemental Table S6.
