
RT-PCR verification of ncRNA predictions. Positive controls include the known small ncRNAs listed in Table 3 as well as eight randomly chosen mRNAs of GENCODE protein-coding genes. Negative controls are randomly selected intergenic and intronic regions. Sets of RNAz and EvoFold predictions were manually selected both overlapping (T+) and not overlapping (T−) with TARs/transfrags. In addition, we selected a set of overlapping RNAz/EvoFold predictions (see Methods).











