Anti-sense example of RACE products. UCSD genome browser graph for prediction 1.4, a region on Chr.11 identified by all four methods. The “Predicted Promoters” track show the regions predicted by any of the four methods. The “Designed Primers” track shows the nested primer pairs used to perform the 5′-RACE experiments. For 5′-RACE, the transcripts are oriented opposite to the primers and end at the nested primer. The “Mapped RACE products” track shows the validated results of sequencing the RACE products. Only the properly oriented RACE products are considered fully valid and the TSSs should be at their 5′-ends (for more details, see Methods). Other standard tracks from the May 2004 (hg17) assembly are shown to give the context of the promoter. Note the empty pseudogene tracks indicating that the identified transcripts are unlikely to be pseudogenes, and the histone modification tracks that constitute a large fraction of the experiments used to make the predictions. Two clusters were found. The cluster on the right appears to be a 3′-UTR anti-sense transcript to GENCODE putative AC051649.7. The cluster on the left appears to be a 5′-UTR anti-sense transcript to a novel gene currently only identified by a GIS-PET.
