Features that distinguish C. intestinalis methylated and unmethylated genes. (A) Histogram showing the frequency of all genes with CpG[o/e] frequencies between 0 and 2. CpG[o/e] of genes whose methylation status was analyzed by bisulfite sequencing are shown in closed (methylated) and open (unmethylated) triangles below. (B) Frequencies of genes that are evolutionarily conserved between C. intestinalis, Homo sapiens, and D. melanogaster plotted against CpG[o/e]. (C) Frequencies of C. intestinalis orthologs of human CpG island genes plotted against CpG[o/e]. (D) Frequencies of C. intestinalis orthologs of human non-CpG island genes plotted against CpG[o/e]. (E) Frequencies of 188 genes that recorded the highest number of EST hits (>500) in the UniGene database plotted against CpG[o/e].
