
Immunohistochemical study of Nr6a1 protein in embryonic day (ED)10.5 mouse embryos. Frozen sections of ED10.5 mouse embryos were fixed by ethanol and incubated either with rabbit anti-Nr6a1 antibodies (A,B,D) or with rabbit IgG isolated from preimmune sera (C,E). Signals were present in somites on both sides of the notochord. The vertical line in the sketch (F) indicates the location of the sections, and the photomicrographs at right (B,D) are magnified images of the boxed regions at left (A).











