Developmental stage-specific decondensation and expression from human Yq12 using the clone 102d(2) (AY598345) from the locus. (A,B) Condensed fluorescence signals from testis and skin sections at 20-wk embryonic stage. (C) Elongated signals (arrow) indicating decondensation of the region at 32 wk of gestation. (E) A testicular section from 36 wk of gestation showing abundant transcripts in nuclei. (G) The expression profile from an adult testicular section, showing the presence of Yq12 complementary RNA in the cytoplasm also. (D,F,H) The corresponding RNase treated sections. (I) The result of hybridization using a nontranscribing 50f2 probe. (J) FISH on to ejaculated sperms with condensed signals in the nuclei. (Bars, 10 μm). (K,L) The developmental stage-specific RNAs and corresponding Northern blot with the Yq12 genomic clone 102d(2) as the probe. (Lanes 1–5) 20-wk testis, 36-wk kidney, 36-wk spleen, 32-wk testis, and 36-wk testis, respectively. (Arrows indicate the positions of 18s and 28s rRNAs). A heterogeneous signal of ∼5–9 kb is obtained only in 36-wk testis, thus indicating a testis-specific and developmental stage-specific expression.
