Identification of novel peptide hormones in the human proteome by hidden Markov model screening

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Figure 4.
Figure 4.

Colocalization of spexin and augurin with insulin in endocrine cells. Flag-tagged NPK, spexin, and augurin were transfected into rat pancreatic cells and fixed cells subjected to double immunofluorescence with Flag and insulin antibodies. (A) Neuropeptide K, (B) spexin, and (C) augurin show colocalization with insulin in small, cytoplasmic punctate structures. In all cases the majority of Flag immunoreactive puncta are also positive for insulin.

This Article

  1. Published in Advance February 6, 2007, doi: 10.1101/gr.5755407 Genome Res. 17: 320-327

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