Primary structure of spexin and augurin. Sequence of spexin and augurin with the signal peptide underlined, pro-hormone cleavage sites boxed, and predicted processed peptide indicated in gray. Arrows indicate where the Flag antigen sequence (DYKDDDDK) was inserted to facilitate immunochemical detection of peptide products. Conservation among orthologs is shown below: (*) identity, (:) high homology; (·) low homology. The C-terminal glycine residue of the predicted spexin peptide is likely to be removed and the peptide amidated, a feature common to known peptide hormones. Both spexin and augurin peptides are enriched in aromatic amino acids.
