Figure 4.
Genes indicating gain of imprinting in some hESC lines. Allele-specific gene expression status was determined by (A) RFLP where the “A” allele contains the restriction site and a band ratio of <1:3 indicates biallelic expression (L, DNA ladder; −, undigested PCR product; +, digested PCR product; N, no reverse transcriptase control) or (B) cDNA sequencing where arrows indicate polymorphic sites.
