Figure 2.
Genes biallelically expressed in all informative cell lines. (A) RFLP analysis of allele-specific expression where the “A” allele contains the restriction site and a band ratio of <1:3 indicates biallelic expression (L, DNA ladder; −, undigested PCR product; +, digested PCR product; N, no reverse transcriptase control). The biallelic expression of SLC22A18 was confirmed by sequencing. (B) Allele-specific expression status of the nonimprinted genes, NAP1L4 and TSSC4. Arrows indicate polymorphic sites.
