Figure 3.
A two-step PCR protocol for genotyping the cea-associated deletion. (A) The NHEJ1 gene with the positions of the two linked SNPs designated by * and primer locations within intron four. (B) Expanded representation of the cea-associated deletion region (outlined by diagonal dashed lines), with flanking and internal primers. (C) Electrophoretogram demonstrating PCR results using primers shown in B on DNA from Normal (N), Carrier (C), and Affected (A) dogs. (Lane 1, M) A marker lane with sizes as indicated. Set I of PCR products (lanes 2–4) presents amplification results using primers NHEJ1-F17 and NHEJ1-R17. Set II (lanes 5–7) presents results using primers NHEJ1-F20 and NHEJ1-R23.
