Figure 4.
Mutational analyses of predicted SOX-binding motifs in the COL9A1 promoter. (A) The proximal promoter region of the COL9A1 human gene showing the conserved and computationally predicted SOX-binding motifs underlined (M1–M7). Mutated nucleotides are indicated by capital letters. RCS cells (B) or NIH3T3 cells (C) were transiently transfected (in triplicates) with the COL9A1-WT promoter construct (p846) or with the p846 construct with mutations (CA to GG) in the predicted SOX-binding motifs M1, M2, M3, M4, M5, M6, and M7.
