Figure 1.
Infection of N2 worms in liquid culture. Synchronized L1 worms were grown in liquid culture with E. coli HB101 for 24 h at 25°C before addition of M. nematophilum; either virulent CBX102 (A) or avirulent UV336 (B) was added. Infection was allowed to proceed for 6 h before worms were harvested for RNA extraction and staining adherent bacteria with the nucleic acid dye SYTO 13 (Molecular Probes). Infection (A) is apparent from the bacterial staining in the rectum and the associated tail swelling. Arrow indicates anus.
